|
Post by Jodie - admin on Jan 21, 2015 16:40:55 GMT
Hey everyone, We've been having some discussion over the difference between spent and resting female gonads and I have some photos for show and tell. Here are our stages for reference (stages were determined from a variety of published and non-published material on C. pagurus): 1 Immature: thin translucent gonad, white or pale 2 Undeveloped: lobes present, grey or pink 3 Developing: gonad developing covers less than 50% of cavity 4 Mature: orange, red, more than 50% of cavity filled with gonad 5 Resting/recovering: white, loose, remnant eggs Has anyone done this work before, we will eventually do some histology as well, but not on all populations so it would be good to know that we are assessing correctly now, rather than later. We are open to advice at this stage! These following pictures we need some feedback on: can you visually determine the difference between resting and recovery if there are no eggs present, prior to histological examination? To hazard a guess the top two look like resting - lobes are well developed but look like they are not in reproductive mode. The bottom figure looks like lobes are empty but the colour and consistency is different so could be recovering? No eggs were observed on/in this female. Comments anyone? J Attachments:
|
|
|
Post by Magnus on Jan 21, 2015 19:54:16 GMT
Happy to section some of these for a definitive answer . . . .
|
|
|
Post by snorreb on Jan 22, 2015 9:31:49 GMT
Looked in our archives from 2003 and found one "early maturation" gonad where we have both macroscopic and microscopic (histology) picture. The gonad was stored in formalin so it is a bit hard to compare with the pictures you got, but it looks similar to the bottom one in your picture. Based on histology it was determined as "Early secondary vitellogenesis" (maturing). Attached the two pictures. (Bit poor quality on the microscope picture, sorry for that).
So my guess for the pictures: The two on top is resting, the bottom one recovery/early maturation.
But i will start out with gonads that look like these when i start my histology work. I have pictures like yours (before gonads were put in formalin) so it will also be easier for us to compare with the sections.
Cheers, Snorre
Attachments:
|
|
|
Post by Jodie - admin on Jan 23, 2015 11:20:16 GMT
Thanks Magnus, we will eventually be doing histology on all our samples. But just trying to get a handle on the visual stages prior to that work.
Thanks for adding pics Snore!
|
|
|
Post by Jodie - admin on Feb 27, 2015 18:19:08 GMT
We've got a whole photo catalogue going now and histology to come. I will be creating a COMPLETE protocol from start to finish on: how to do a study on size at maturity for brown crab. Including lab and histology proceedures and R code for analysing the data.
Stay tuned, this won't be ready until after we publish the collaborative size at maturity stuff but it is coming!
Why I think it's important: because I don't believe in this "I had to figure it out myself so, so do you" nonsense. I would like to see a comprehensive protocol available for ANYONE to do this study. It would be great to see industry led investigations into regional size at maturity for brown crab in the years to come, this protocol will enable that to happen.
If anyone wants to contribute to the details of the protocol they are more than welcome.
Thanks, Jodie
|
|
|
Post by Jodie - admin on Jun 4, 2015 14:46:58 GMT
Histology work complete and interpretation to follow shortly!
|
|